Vitamin D vs Vitamin D3

"Vitamin D" on a supplement label is almost always vitamin D3 (cholecalciferol), but "vitamin D" as a chemistry term is an umbrella including both D3 and D2 (ergocalciferol). Here's what actually differs.

Attribute Vitamin D3 (cholecalciferol) Vitamin D2 (ergocalciferol)
OriginAnimal skin from 7-DHC + UVB; fatty fish; egg yolkYeasts/fungi from ergosterol + UV; UV-exposed mushrooms
ChemistrySide chain has H at C24Side chain has methyl at C24; double bond at C22–C23
Effect on serum 25(OH)DRaises by ~1 ng/mL per 100 IU/day~60–90% less effective per equivalent dose (Tripkovic 2012)
Half-life of the metabolite25(OH)D3 ~15–25 days apparent; ~60 d terminal25(OH)D2 shorter
Common supplement sourceLanolin (sheep wool) or lichen (vegan)Yeast or fungi (vegan)
Prescription formsMost European & ROW prescription vitamin DUS prescription 50,000 IU capsules (Drisdol)
RecommendationPreferred by most modern guidelinesReasonable for vegans who avoid lichen-D3; ask about substitution

Why the effectiveness gap?

Both forms are 25-hydroxylated by CYP2R1 in the liver. But D2 is less avidly bound by vitamin D-binding protein (DBP), cleared faster, and its metabolites are catabolised more rapidly. Net result: D2 produces smaller and shorter-lived rises in serum 25(OH)D.

If your prescription is ergocalciferol (Drisdol, "vitamin D2"), it's worth asking your prescriber whether cholecalciferol at the same IU dose would suit you better — most will say yes.

Reading a supplement label

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